Lgr5 Egfp Ires Creert2, supplied by The Jackson Laboratory, used in various techniques. Bioz Stars score: 89/100, based on 50 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more

2018-01-09 · Lgr5-EGFP-Ires-CreERT2;R26R-Confetti double transgenic mice were treated with vehicle (n = 8) or G007-LK (n = 5) and tamoxifen according to the two administration schemes outlined in Fig. 1d, f. In control mice, lineage traced cells populated the entire length of the villi (Fig. 2 a) in the majority (69.9%) of the villi (Additional file 3 : Figure S2A).

Lgr5-EGFP-ires-creERT2 mice were crossed with APCfl/fl mice. Cre enzyme activity was induced by intraperitoneal injections of tamoxifen (2 mg/mouse). Murine small intestines with Lgr5-eGFP-IRES-CreERT2 (Lgr5-Cre) mice. To this end, tamoxifen (Tam) was administered to 8-10-week-old mice. After three consecutive days of Tam treatment, double immunofluorescence staining showed that some Lgr5+ antral stem cells lacked E-cadherin expression in Lgr5-Cre;Cdh1fl/fl mice (Figure 2A). 南模生物.

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Expression of Bmi1, another putative N et al. Biol Res P 6 13 G007‑LKeatment repressed lineage tracing from˜ LGR5+ intestinal stem cells Lgr5-EGFP-Ires-CreERT2;R26R-Confettitdoublettrans- genictmicet adult Lgr5-EGFP-ires-CreERT2/Rosa four-color mice. Confocal. analyses of the clonal output were performed at 14 days, 2 months, 6 months, and 12 months after the final tamoxifen. Long-term culture of adult progenitor cells in 3D is a recently emerging technology that inhabits the space between 2D cell lines and organ slice culture. Adaptations to defined media components in the wake of advances in ES and iPS cell culture has led to the identification of conditions that maintained intestinal cell progenitors in culture.

Apr 25, 2012 Mice lacking TLR4 in Lgr5-positive intestinal stem cells were gener- ated by first crossing the Lgr5-EGFP-IRES-creERT2 mice (12) with mT/mG.

To delete exons 2–10 of Trp53 in colonic stem cells, Trp53 flox/flox mice (Jackson, 008462) or Rosa26-LacZ mice (Jackson, 003474) were crossed with Lgr5-EGFP-IRES-creERT2 mice (Jackson, 008875; ref. 15). All mice were crossed onto a C57BL/6 background for at least 10 generations. Transcriptional differences between ISCs and their daughter cells can be explored by use of the Lgr5‐EGFP‐ires‐CreERT2 knock‐in (Lgr5‐ki) mouse (Supplementary Figure S1A; Barker et al, 2007).

To assess whether all epithelial tumours of the gastrointestinal tract are derived from LGR5-positive stem cells, we crossed Lgr5-EGFP-IRES-creER(T2) mice, which express EGFP and Cre recombinase driven by the Lgr5 promoter, with CEA424-SV40-TAg mice, which develop pyloric neuroendocrine carcinomas of epithelial origin.

Tumors were then flow-sorted into fractions of epithelial cells that expressed high or low levels of Lgr5 and were molecularly characterized using gene expression profiling and array comparative genomic hybridization. 2018-02-02 · Lgr5-eGFP-IRES-CreERT2 mice were crossed with B6.Cg-Gt(ROSA)26Sortm9(CAG-tdTomato)Hze/J mice (Jackson Laboratories) to generate the Lgr5-eGFP-IRES-CreERT2; Rosa26-CAG-tdTomato heterozygote.

Se hela listan på blog.csdn.net Lgr5-EGFP-IRES-creERT2基因敲入工具鼠 索取资料 欢迎您在此索取产品资料，联系订购产品，提交后您的需求信息将立即自动发送到供应商的E-mail邮箱，同时也将抄送一封到您的邮箱，感谢您的惠顾！ with WT Lgr5-EGFP-ires-creERT2 mice and by multiple generation selective breedings obtaining mice that were WT, CD44 /,or TLR4 / and heterozygous for the Lgr5-EGFP-ires-creERT2 re-porter.
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Heterozygous mice harbor an Lgr5- EGFP-IRES-creERT2 "knock-in" allele that both abolishes Lgr5 gene function  were generated by crossing Lgr5–EGFP–ires–CreERT2 and.

实验动物商城平台. 在线客服 ; 实验动物一站式采购平台 cells in Lgr5-eGFP-IRES-CreERT2 mice. In contrast, only 1.7 ± 0.30% of Bmi1+ ISCs, identified by the crypt Rosa-YFP+ signal after 1.5-d tamoxifen exposure in Bmi1-CreER; Rosa26-YFP mice, incorporated EdU (Fig. 1 A–F and N). To examine the relative contribution of Lgr5 vs.
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Lgr5-EGFP-ires-creERT2 mice,11 APCfl/fl mice,25 Axin2-lacZ mice,26 and C57B/6 wild-type mice (6–12 weeks old) were used for experiments. Lgr5-EGFP-ires-creERT2 mice were crossed with APCfl/fl mice. Cre enzyme activity was induced by intraperitoneal injections of tamoxifen (2 mg/mouse). Murine small intestines

To delete exons 2–10 of Trp53 in colonic stem cells, Trp53 flox/flox mice (Jackson, 008462) or Rosa26-LacZ mice (Jackson, 003474) were crossed with Lgr5-EGFP-IRES-creERT2 mice (Jackson, 008875; ref. 15). All mice were crossed onto a C57BL/6 background for at least 10 generations. Transcriptional differences between ISCs and their daughter cells can be explored by use of the Lgr5‐EGFP‐ires‐CreERT2 knock‐in (Lgr5‐ki) mouse (Supplementary Figure S1A; Barker et al, 2007). In this mouse model, GFP expression is driven from the Lgr5 locus, leading to highest GFP levels in Lgr5 + cells (GFP high).